GSK3A Knockout cell line (THP-1)

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GSK3A Knockout cell line (THP-1) Order

GSK3A Knockout cell line (THP-1)
Catalog#
YKO-HT0491
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSK3A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSK3A Knockout cell line (THP-1)
Species
Human
Gene Name
GSK3A
Gene ID
2931
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript GSK3A-201)
Validation: PCR+Sanger Sequencing
GSK3A Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSK3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSK3A gene knockout GSK3A Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSK3A Knockout cell line (THP-1)
GSK3A Knockout cell line (THP-1)
Catalog#
YKO-HT0491
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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EZ-cryo™ cell freezing medium

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  • GSK3A Knockout cell line (Hep G2)

    GSK3A Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-HT3383
    YKO-HT3383(Hep G2)
  • GSK3A Knockout cell line (HEK293)

    GSK3A Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-H841
    YKO-H841(HEK293)
  • GSK3A Knockout cell line (HeLa)

    GSK3A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-XN27236
    YKO-XN27236(HeLa)

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EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • GSK3A Knockout cell line (Hep G2)

    GSK3A Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-HT3383
      inquiry   More details
  • GSK3A Knockout cell line (HEK293)

    GSK3A Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-H841
      inquiry   More details
  • GSK3A Knockout cell line (HeLa)

    GSK3A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-XN27236
      inquiry   More details

GSK3A Knockout cell line (THP-1) Order

GSK3A Knockout cell line (THP-1)
Catalog#
YKO-HT0491
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
GSK3A Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
GSK3A Knockout cell line (THP-1)
Species
Human
Gene Name
GSK3A
Gene ID
2931
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript GSK3A-201)
Validation: PCR+Sanger Sequencing
GSK3A Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the GSK3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, GSK3A gene knockout GSK3A Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
GSK3A Knockout cell line (THP-1)
GSK3A Knockout cell line (THP-1)
Catalog#
YKO-HT0491
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

KO Cell Lines

  • GSK3A Knockout cell line (Hep G2)

    GSK3A Knockout cell line (Hep G2)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-HT3383
    YKO-HT3383(Hep G2)
  • GSK3A Knockout cell line (HEK293)

    GSK3A Knockout cell line (HEK293)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-H841
    YKO-H841(HEK293)
  • GSK3A Knockout cell line (HeLa)

    GSK3A Knockout cell line (HeLa)
      inquiry   More details

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-XN27236
    YKO-XN27236(HeLa)

Related Popular Products

Wild-type Cell Lines

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium
      inquiry   More details

KO Cell Lines

  • GSK3A Knockout cell line (Hep G2)

    GSK3A Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-HT3383
      inquiry   More details
  • GSK3A Knockout cell line (HEK293)

    GSK3A Knockout cell line (HEK293)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-H841
      inquiry   More details
  • GSK3A Knockout cell line (HeLa)

    GSK3A Knockout cell line (HeLa)

    Organism: Human
    Gene Name: GSK3A
    Gene ID: 2931
    Size: 1*10^6
    Catalog#: YKO-XN27236
      inquiry   More details

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